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Briefly, all reactions were performed in 3,3-dimethylglutaric acid (DMG) buffer ([pH 7.0] 3,3-dimethyl glutarate 50 mM, DTT 2 mM,EDTA 1 mM, and the ionic strength was adjusted to 0.15 M) at 37 °C.

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Control reactions were performed by substituting the lectins with TBS-T-BSA. For ConA, an additional control reaction was also performed by …

The objective of this study was to determine the phytochemical content, antibacterial activity and antioxidant activity of leaves, bark and pods of Acacia nilotica.The different extracts of acacia were evaluated for total phenolic, flavonoid and protein contents, antibacterial (agar well diffusion and broth dilution methods) and antioxidant (DPPH; 1,1 …

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Glycoside bond formation in the eyes of the majority of chemists is still closely connected to the names of Koenigs and Knorr, who invented the reaction in 1901. Ever since it has been proven useful in most every-day applications, however, some peculiarities in the nature of the target molecule render this tool less useful for constructing 2-deoxy glycosides.

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Segregation of homologs—rather than sister chromatids—is unique to meiosis I. Galander et al. show that the meiosis-I-specific Spo13 protein prevents sister chromatid segregation by controlling the effects of multiple kinases to both enforce sister kinetochore co-orientation and prevent premature loss of cohesion.

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